Fitness First in Clough Road, Hull, is closing.
The company has confirmed the gym will open for the last time next month.
The move, which affects more than 2,000 members, will result in nine job losses.
A spokesman for the chain said: "We can confirm that Fitness First Hull is to be closed on 4th March as a result of the group restructuring announced at the beginning of June last year."
For more on this story, see tomorrow's Hull Daily Mail.
