The Cross-Country Handicap Chase was won by Big Shu.
Second was Shakervilz, third was Outlaw Pete and fourth was Bostons Angel.
The race was postponed because of an injury to JT McNamara.
The renowned amateur rider was partnering Galaxy Rock for trainer Jonjo O'Neill in the Fulke Walwyn Kim Muir Challenge Cup when the pair crashed out at the first fence.
Medical staff were quickly on hand to treat McNamara and it was decided to transfer him to Frenchay Hospital in Bristol for further treatment without delay.
Course spokesman Andy Clifton said: "JT McNamara is being transferred to Frenchay Hospital in Bristol via air ambulance and continues to undergo assessment."
In the first race of the day, Benefficient, in the hands of Brian Cooper, won the Jewson Novices' Chase, the first race of St. Patrick's Thursday.
This is Cooper's first ever Cheltenham Festival win.
Holywell, ridden by Richie McLernon, was a deserving winner in the Pertemps Final at 2.05, seeing off Captain Sunshine and Jetson.
A P McCoy's Shutthfrontdoor was fourth, while pre-race favourite Sam Winner ridden by Ruby Walsh could not make his mark on the race.
Cue Card ridden by Joe Tizzard and tipped by Herbert, sailed over the jumps to win the 2.40 RyanAir Chase ahead of of pre-race favourite First Lieutenant, ridden by today's first winner Bryan Cooper.
Third placed was For Non Stop, while Riverside Theatre, owned by actor James Nesbitt, finished fourth.
Solwhit, in the hands of Paul Carberry, won the World Hurdle and a record £275,000 in prize money two and a half lengths clear of Celestial Halo in second.
Smad Place was third, after a photo finish.
Reve du Sivola, tipped by Herbert, was fourth while pre-race favourite Oscar Whisky, trained by Nicky Henderson did not feature much to the disappointment of race-goers
A 50-1 stunner, Carrickboy kicked clear away with two to jump to win the 16.00 Byrne Group Plate, in the hands of Liam Treadwell.
Herbert's choice Vino Griego ridden by Jamie Moore was suited to the softer ground and finished second with a great effort to close the winning horse down.
Tartak was third and Hunt Ball was fourth.
In the most dramatic, thrilling finish of the day, Same Difference snatched victory on the line from the bookies favourite Super Duty for the 4.40 Fulke Walwyn Kim Muir Challenge Cup.
With Derek O'Connor on board, Herbert's next best bet of the day finished second while Nina Carberry, who came so close on Tolfino Bay yesterday, was riding Romanesco who finished third. Alfie Sherrin finished fourth.
Paddy Brennan has been banned for nine days (March 28, 30, 31 and April 1-6), ruling him out of the Grand National meeting at Aintree, for using his above the permitted level on Captain Sunshine in the Pertemps Final.
Robbie Power was banned for two days (March 28 and 30) for the same offence aboard Jetson in the same race.
